Experiment 2 Bioprocess Tech ...damn boring... O.o wahaha

For this experiment…its just for all of us to learn how to prepare a bioreactor, make MEDIA(again..for hafiz and i) for both scale-up fermentation and seed culture.

To make 2Litres of media…we just added 50g of LB powder and topped it up to 2Litres…making sure no foams are form as we were told that the presence of foam would result in the loss of media and cells…

a top down view while we were trying desperately to mix the powder with distilled water?...doesn't it look like the opp of an egg?..(white yolk..yellow egg 'white') LOLS

Then, we separated 100ml into a flask to be inoculated with our bacteria which were on a LB-agar plate and incubated it overnight while the remaining 1900ml was poured into the fermenter and AUTOCLAVED TOGETHER.. ALL AT THE SAME TIME..
wow….its a wonder nothing blew up.. wahaha..

Ok… and as for preparing the fermenter itself…we didn’t really do much…it was more like..the teacher and the DT doing it..so I ain’t gonna copy and paste la hor.. XD

Done by Killa